Generator
LacI:Cpn60

Part:BBa_K538201:Design

Designed by: Bas Stringer   Group: iGEM11_Amsterdam   (2011-09-08)

pLacI + RBS + Cpn60

LacI:Cpn60
K538201


This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into E. coli, it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (pLacI: BBa_R0010) so that it may be expressed constitutively in LacI-null strains, or inducibly in LacI-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as BBa_K538001.

For more information about the subparts' purpose and design, refer to their main page or design page, respectively.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1096
    Illegal BamHI site found at 1162
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]